Curcumin |
Source:
http://www.cancerprev.org/Meetings/2004/Symposia/795/133
Curcurmin induce apoptosis in human cervix epithelioid
carcinoma cells (HeLa) through activation of caspase- 3 and P53
C-S Yu PhDa, JK-S Chan Bsb, J-G Chung PhDc
a“, Center of General Education, cDepartment of Microbiology; China
Medical University, Taichung 404, Taiwan, bDepartment of Biochemistry,
University of British Columbia, Vancouver, BC, Canada “
Curcurmin has been demonstrated in rats to prevent azoxymethane-induced
aberrant crypt foci, putative precursor lesions in the colon and in mice
to prevent tumorigenesis induced by azoxymethane in the colon and by
N-ethyl N-nitrosoguanidine in the duodenum and forestomach. The
mechanism of curcurmin affect on human cervix cancer cells was not
addressed. Therefore, curcurmin was used to study the biological
activities on the human cervix epithelioid carcinoma cells (HeLa). On
MTT assay, curcurmin showed obvious cytotoxic effects and inhibit the
proliferation of HeLA cells. The cytotoxic effects of curcurmin was
accompanied by the dose- and time-dependent appearance of
characteristics of apoptosis including DNA fragmentation (gel
electrophoresis) and sub-G1 ratio (flow cytometric assay). The HeLA
cells cotreated with curcurmin caused a rapid transient induction of
caspase-3 activity, but not caspase-1 activity. We also found out that
cleavage of poly(ADP-ribose) polymerase (PARP) and decrease of
pro-caspase-3 protein were detected in curcurmin-treated HeLa cells.
Increased the pro-apoptotic protein (bax), cytochrome C release and
caspases-9 and 3 activity and decreased anti-apoptotic protein (Mcl-1)
were detected in HeLa cells after cotreated with curcurmin. We also used
PCR and multiplex PCR methods to examine the caspase 3 gene expression
and the results show that curcurmin induced caspase-3 gene expression.
Paper presented at the International Symposium on Predictive Oncology
and Intervention Strategies; Nice, France; February 7 - 10, 2004; in
poster session 795 (Apoptosis mechanisms).
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