Main Navigation

 

 

Curcumin

 

Source: http://www.cancerprev.org/Meetings/2004/Symposia/795/133


Curcurmin induce apoptosis in human cervix epithelioid carcinoma cells (HeLa) through activation of caspase- 3 and P53  C-S Yu PhDa, JK-S Chan Bsb, J-G Chung PhDc

a, Center of General Education, cDepartment of Microbiology; China Medical University, Taichung 404, Taiwan, bDepartment of Biochemistry, University of British Columbia, Vancouver, BC, Canada

Curcurmin has been demonstrated in rats to prevent azoxymethane-induced aberrant crypt foci, putative precursor lesions in the colon and in mice to prevent tumorigenesis induced by azoxymethane in the colon and by N-ethyl N-nitrosoguanidine in the duodenum and forestomach. The mechanism of curcurmin affect on human cervix cancer cells was not addressed. Therefore, curcurmin was used to study the biological activities on the human cervix epithelioid carcinoma cells (HeLa). On MTT assay, curcurmin showed obvious cytotoxic effects and inhibit the proliferation of HeLA cells. The cytotoxic effects of curcurmin was accompanied by the dose- and time-dependent appearance of characteristics of apoptosis including DNA fragmentation (gel electrophoresis) and sub-G1 ratio (flow cytometric assay). The HeLA cells cotreated with curcurmin caused a rapid transient induction of caspase-3 activity, but not caspase-1 activity. We also found out that cleavage of poly(ADP-ribose) polymerase (PARP) and decrease of pro-caspase-3 protein were detected in curcurmin-treated HeLa cells. Increased the pro-apoptotic protein (bax), cytochrome C release and caspases-9 and 3 activity and decreased anti-apoptotic protein (Mcl-1) were detected in HeLa cells after cotreated with curcurmin. We also used PCR and multiplex PCR methods to examine the caspase 3 gene expression and the results show that curcurmin induced caspase-3 gene expression.

Paper presented at the International Symposium on Predictive Oncology and Intervention Strategies; Nice, France; February 7 - 10, 2004; in poster session 795 (Apoptosis mechanisms).

 

 

 

 
The Power Hour:
Show-Schedule
(7-10am CST)
Listen Live

Listen FREE thru Global Star Satellite Feed

 

 

 
 
 

 

   

 
         


All logos and trademarks in this site are property of their respective owner.
FAIR USE NOTICE: This site contains copyrighted material the use of which has not always been specifically authorized by the copyright owner. We are making such material available in our efforts to advance understanding of environmental, political, human rights, economic, democracy, scientific, and social justice issues, etc. We believe this constitutes a 'fair use' of any such copyrighted material as provided for in section 107 of the US Copyright Law. In accordance with Title 17 U.S.C. Section 107, the material on this site is distributed without profit to those who have expressed a prior interest in receiving the included information for research and educational purposes. For more information go to: http://www.law.cornell.edu/uscode/17/107.shtml

Copyright © 2007. The Power Hour. All rights reserved.